FROSTY
Untersuchung der Rolle der CBF Transkriptionsfaktoren in der Frosttoleranz von Arabidopsis und Eukalyptus - Ansätze zum molekularen Verständnis der Signaltransduktion und Erstellung von molekularen Markern für die Züchtung
Projektbeschreibung
Much of the work on freezing tolerance has focused on the CBF pathway, which regulates about 20% of all cold-regulated genes. CBF genes appear to be ubiquitous in plants and are always present in multiple copies. However, it still remains to be determined how their expression is regulated and what the precise contribution of each individual gene to freezing tolerance and cold acclimation is. The general objective of this project is twofold: 1) to better understand the regulation of CBF gene expression and the involvement of the CBF cold response pathway in freezing tolerance and 2) to examine other pathways involved in freezing tolerance. We are using Arabidopsis and a commercially important tree species, Eucalyptus. For the first objective, we use three complementary approaches: i) the study of natural variation, ii) the use of reporter-genes as well as RNAi and over-expressing lines, and iii) the study of mutants affected in CBF regulation. For the second objective, we will perform QTL mapping in Arabidopsis.
We have taken an approach that relies on the natural variation present between different accessions of wide eco-geographic origins. A phenotyping by elektrolyte leakage analysis of an Arabidopsis core collection of 48 accessions defined by the EPGV and complemented by 7 additional accessions is in progress. These data will allow us for the first time to judge the extent of natural genetic variability in freezing tolerance in any plant species. The CBF genes of these accessions have already been sequenced at the EPGV. These data will be used to analyze correlations between sequence polymorphisms, gene expression and freezing tolerance phenotypes. Finally, these results will allow the identification of more efficient variants of CBF genes and promoters. A similar approach is currently also used with a collection of Eucalyptus ecotypes from Tasmania. In parallel, we are generating RNAi lines deficient in the expression of either specific CBF genes or of all three genes for detailed physiological and molecular analysis.
The results we expect to obtain from this project will constitute an important step for better applying our knowledge of the CBF genes, and perhaps others, to improve the freezing tolerance of many crop plants.
Partner
Dr. Dirk K. Hincha, Dr. Ellen Zuther
Max Planck Institute of Molecular Plant Physiology
Am Mühlenberg 1
14476 Potsdam
e-mail: hincha@mpimp-golm.mpg.de
Julio Salinas
Departamento de Biología de Plantas
CIB-CSIC
Madrid
Spain
Chantal Teulieres
Universite Paul Sabatier
Castanet Tolosan
France
Evelyne Teoule
SGAP
INRA
Versailles
France
Prof. Heather McKhann
EPGV
INRA-CNG
Evry
France
FROSTY
INTERNATIONAL01.04.2007 - 31.03.2010
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