YelLowSin'

Projektbeschreibung

New varieties of oilseed rape (Brassica napus) that combine the yellow seed trait with low levels of anti-nutritive sinapine would represent a highly significant new product that would considerably raise the value of rapeseed meal as a protein source for both animal feed and human nutrition. This project will combine conventional breeding strategies with molecular marker techniques, mutagenesis (TILLING, and transgenic approaches to develop new varieties of oilseed rape (winter type) and canola (spring rape) with improved nutritional value of the seed meal. Cooperation with Canada is a unique opportunity for complementary research because Canadian canola breeders and German oilseed rape breeders have similar aims but different material. Furthermore, plant material as well as molecular genetic and genomic tools for B. napus can be shared between both countries. By exchanging plant material and molecular markers with our partners in Canada we will perform allelic screens of different Canadian and German breeding material. This will allow the identification, integration and combination of novel genetic variability for low anti-nutritive substances in Canadian spring canola and German winter oilseed rape varieties.

Project Aims:
The overall aim of this joint project is the development and use of molecular genetic and functional genomics tools to assist in the breeding of novel yellow-seeded, low sinapine oilseed rape varieties as a new, high-value protein source for animal feed and human nutrition. Building on existing cooperations with Canadian partners involved in canola breeding and B. napus functional genomics, this aim will be achieved via three complementary strategies:

a) Development of molecular markers closely linked to relevant genes that reduce the contents of sinapine, tannins and anti-nutritive fibre fractions (ADF, NDF, ADL), by combining genetic fine mapping with phenotypic screening of suitable segregating germplasm, including the development and use of assays for high-throughput screening of anti-nutritive substances;

b) Breeding of stable yellow-seeded oilseed rape lines by conventional and marker-assisted breeding;

c) Development of conventional, mutation-induced and transgenic yellow seeded low sinapine rapeseed by marker-assisted breeding, TILLING in a yellow-seeded EMS population for sinapine mutants, and transformation of homogeneous yellow-seeded lines with dsRNAi cassettes for silencing of genes involved in the sinapine pathway.

Die Erfolgsgeschichte Raps geht weiter

Rod Snowdon und Wolfgang Friedt

Artikel zum Projekt YelLowSin', erschienen im GenomXPress 2.08 sowie als Sonderdruck zu den DLG-Feldtagen und zur Biotechnica 2008.

Gedruckte Exemplare können über die GABI Geschäftsstelle bezogen werden.

GenomXPress 2.08 Sonderdruck YelLowSin' (PDF)